Figure 5

Actin related genes containing one or more CREB and E2F1 binding sites stratify in expression versus occurrence of transcription factor binding sites; a subset of actin related genes are differentially expressed in DN. Actin related genes were extracted from each gene set for each condition by gene ontology (GO) annotation search. For each actin related gene, the occurrence of CREB and E2F1 sites were determined in the 5000 bp region upstream of the transcription start site (TSS). A. The genes are arranged as fold change in expression for the co-treated dataset versus the control dataset. B, C. The occurrence of CREB and E2F1 sites were determined, and appear to stratify with change in gene expression, suggesting functional consequence for the presence of these transcription factor binding sites with respect to the change in gene expression induced by TGFβ and CCN2. D. A subset of actin related genes from the ERCB biobank illustrate differential gene expression in biopsy samples from patients with DN versus controls (genes differentially expressed at p < 0.05).