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Figure 5 | BMC Genomics

Figure 5

From: Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes

Figure 5

Small RNA populations in the non-virulentE. histolyticaRahman strain. (A) Distribution of small RNA reads cloned from a size-fractionated small RNA library from the non-virulent E. histolytica Rahman strain. (B) Small RNA length distribution for reads in the Rahman library (dashed black line: all reads after filtering out those that originate from structural RNAs and repetitive elements reads; solid black line: reads that align to the reference genome). (C) Nucleotide frequency at each position for the aligned 27nt reads in the Rahman library reveals a 5-G predominance. (D) Antisense small RNAs from the E. histolytica Rahman strain map to EhSTIRP1 (EHI_025700). Small RNA reads from HM-1:IMSS EhAGO2-2 IP library and Rahman size-fractionated small RNA library are shown in red bars (small RNAs in antisense direction) and blue bars (small RNAs in sense direction); black arrow represents EHI_025700 and its direction. Insert figures are Northern blot analysis using total RNA samples from both strains to show the presence of antisense small RNAs in the E. histolytica Rahman strain but not in HM-1:IMSS strain for selected EhSTIRP1 probe. Control probe (EHS-D-A16-1) detected small RNA signal in both samples.

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