Procedures | Output |
---|---|
5′-P independent cloning (5′CIP+PNK method) | HM-1:IMSS AGO2-2 IP library |
Total reads by Pyrosequencing | 362,445 reads |
Primer trimming, size-limiting (15–40nt) | 340,280 reads |
Unique sequences (percentage) | 209,513 unique sequences (158,904 cloned only once; 75.8%) (50,609 cloned more than once; 24.2%) |
Scan for tRNA, rRNA | tRNA: 1,640 sequences rRNA: 3,456 sequences |
Scan for SINE/LINE, EhERE elements | 8,073 sequences |
Map to E. histolytica genome | 140,943 sequences |
Map to E. histolytica predicted ORFs | 100,190 sequences |