Figure 2

Distribution of H4K5ac in the genome and its profile relative to the TSS. (A) Distribution of reads from H4K5ac with respect to intragenic regions including promoter, CDS (introns and exons) and intergenic regions in the hippocampus after CFC compared to (B) mock IgG-IP. Promoters were defined as 5 kb upstream of the TSS, the CDS as regions between the TSS and TTS, and intergenic as regions excluding gene bodies. Reads spanning the transcription start site that match both a promoter and transcript are counted twice, thus, leading to total reads greater than 100%. (C) Profile of reads for H4K5ac (red) and IgG-IP controls (green) across ± 5 kb relative to the TSS (left) and spanning the CDS as a percentage of reads averaged over all genes (right). (D) Distribution of reads for H4K5ac and (E) IgG-IP by genomic regions in the absence of CFC (Control). (F) Profile of reads for H4K5ac (red) or IgG-IP (green) in Control. Distribution profiles for H4K5ac after 2 days CFC (red) compared to (G) H4K12ac immediately after 1 CFC session (green) [4]. Window analyses were obtained using EpiChIP [48].