Influence of the individual number of alleles (A), and the lowest read frequency (B) on the minimum number of reads required to determine a complete genotype with a 99.9% confidence level. Each point represents a different individual (N = 36). The minimum number of reads required was a posteriori computed (T1Resampled) by resampling both amplicon replicates (T1Resampled1, T1Resampled2) and the maximal value was retained. The dashed line in (A) illustrates T1Galan Simul computed according to Galan’s et al.  definition of an allele’s dropout (i.e. an allele associated with two reads or less; see text for details).