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Figure 6 | BMC Genomics

Figure 6

From: MHC genotyping of non-model organisms using next-generation sequencing: a new methodology to deal with artefacts and allelic dropout

Figure 6

Influence of the individual number of alleles (A), and the lowest read frequency (B) on the minimum number of reads required to determine a complete genotype with a 99.9% confidence level. Each point represents a different individual (N = 36). The minimum number of reads required was a posteriori computed (T1Resampled) by resampling both amplicon replicates (T1Resampled1, T1Resampled2) and the maximal value was retained. The dashed line in (A) illustrates T1Galan Simul computed according to Galan’s et al. [13] definition of an allele’s dropout (i.e. an allele associated with two reads or less; see text for details).

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