Figure 5
From: Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals
Expression of an assembled contig in HEK293 mammalian cells yields fluorescence. An open reading frame of contig 19888 from Fav2 was synthesized using mammalian preferred codon usage (887 bases of s62Contig19888) and subcloned into pcDNA 3.1, and transfected into HEK293 mammalian cells using Fugene (Boehringer-Mannheim). The left panel depicts a phase contrast image of transfected HEK293 cells, and the right panel depicts fluorescence (using FITC excitation and emission) from the same field. Scale bar = 100 microns.