Figure 5
XopAF contributes to the growth of Xcaw12879 strain in planta . XccA306 (A), Xcaw12879 (Aw), Xcaw12879ΔxopAF (AwΔxopAF), Xcaw12879ΔxopAF- 53:xopAF (AwΔAF:53:xopAF, complement strain), Xcaw12879ΔavrGf1 (AwΔavrGf1), Xcaw12879ΔavrGf1-34:avrGf1 (AwΔGf1:34:avrGf1, complement strain), Xcaw12879ΔxopAF ΔavrGf1 (AwΔxopAFΔavrGf1) and Xacw12879 ΔxopAF ΔavrGf1-34:avrGf1-53:xopAF (AwΔGf1ΔAF:34:avrGf1:53:xopAF, complement strain) were inoculated at approximately 106 cfu/ml concentration into A. Duncan grapefruit, B. Mexican lime and C. Valencia sweet orange leaves using a needleless syringe. Bacterial cells from the inoculated leaves were recovered at different time-points, diluted and counted to plot the growth curve. The values at each time point represent means of three replicates. Means ± SD are plotted.