Verification of differential expression of hemostatic genes by qRT-PCR analysis. The abundance of six genes associated with blood coagulation was determined by quantitative reverse transcription PCR (qRT-PCR) analysis. Data points represent Least Squares Means (LSMEANS; n = 4 birds/genotype) of normalized expression values generated by the general linear models (GLM) procedure in Statistical Analysis System (SAS) software. A two-factor (genotye and age) analysis of variance (ANOVA) was used to determine significance (P≤0.05). The shaded box in each panel indicates significant effects of age (A), genotype (G) and/or the A x G interaction; the parenthesis shows the common standard error (SE) of LSMEANS for that gene as determined by the GLM procedure in SAS.