Verification of differential expression of adipokines by qRT-PCR analysis. The abundance of eight adipokines was determined by quantitative reverse transcription PCR (qRT-PCR) analysis. Data points represent LSMEANS (n = 4 birds/genotype) of normalized expression values. A two-factor ANOVA was used to determine significance (P≤0.05). The shaded box in each panel indicates significant effects of age (A), genotype (G) and/or the A × G interaction; the parenthesis shows the common standard error (SE) of LSMEANS for that gene determined by the GLM procedure in SAS.