Upstream regulators of gene transcription in abdominal fat of juvenile FL and LL chickens. Ingenuity® Upstream Regulator Analysis revealed a large number of transcriptional regulators (see Table 4) controlling lipid metabolism genes in abdominal fat (A). This IPA analysis shows “up-stream regulators” and their downstream targets found among DE fatty acid metabolism genes identified in abdominal fat of the FL and LL chickens. Differentially expressed gene targets regulated by six additional transcription factors are shown (B). The IPA prediction of activation (orange lines and symbols) or (blue lines and symbols) inhibition states is based on prior knowledge accrued by Ingenuity® Knowledge Base and expression values of differentially expressed genes identified by microarray analysis of abdominal fat in juvenile FL and LL chickens. Gene symbol color indicates higher expression in the FL (red) or higher expression (green) in the LL.