Transcriptional analysis of abdominal fat in genetically fat and lean chickens reveals adipokines, lipogenic genes and a link between hemostasis and leanness

Table 2 Top biological functions of DE genes in abdominal fat of juvenile FL and LL chickens*

Diseases and disorders	P-value	# Genes
Developmental disorder	2.76E-07	33
Hereditary disorder	3.01E-06	71
Inflammatory disease	7.14E-06	11
Organismal injury and abnormalities	4.51E-05	31
Metabolic disease	4.77E-05	41
Molecular and cellular functions
Lipid metabolism	6.06E-05	46
Small Molecule biochemistry	6.06E-05	43
Physiological system development and function
Hematological system development and function	1.87E-05	34
Organ morphology	2.56E-05	7
Renal system development/function	2.56E-05	10
Embryonic development	1.06E-04	23
Cardiovascular system function	1.75E-04	8
Top canonical pathways	P -Value	Genes †	Ratio †
Coagulation system	2.56E-08	(7/38)	0.184
Intrinsic prothrombin activation pathway	1.75E-04	(6/34)	0.176
Extrinsic prothrombin activation pathway	4.65E-04	(3/20)	0.15
Acute phase response signaling	5.11E-08	(15/178)	0.08

*Ingenuity® Pathway Analysis (IPA®) software was used for functional annotation and mapping of the DE genes to canonical (metabolic/regulatory) pathways, gene interaction networks, and interactive networks of transcriptional factors that regulate differential expression of target genes. The significance of representation (P-value) is determined by IPA based on the number of DE genes (# Genes) found in each biological category divided by the number of known genes assigned to that category by the Ingenuity® Knowledge Base [42]. The bottom panel shows the significance of the representation of DE genes in canonical pathways by IPA software. The “Genes†” and “Ratio†” columns indicate the number of observed DE genes divided by total number of genes assigned to each canonical pathway by the Ingenuity® Knowledge Base.

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