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Table 3 Functional categories of DE and prior candidate genes expressed in abdominal fat and the average fold change (FL/LL) as determined by microarray and/or qRT-PCR analyses

From: Transcriptional analysis of abdominal fat in genetically fat and lean chickens reveals adipokines, lipogenic genes and a link between hemostasis and leanness

Functional category Symbol Gene name Microarray (FC)* qRT-PCR (FC)*
Hemostasis A2M Alpha-2-macroglobulin −1.89 −1.10
  AGT Angiotensinogen 1.20 -
  ANG Angiogenin −2.51 -
  CFB Complement factor B −1.49 -
  CPB2 Carboxypeptidase B2 −1.43 -
  CPM Carboxypeptidase M −1.32 -
  F2 Thrombin −1.85 −1.35
  F9 Christmas factor −1.51 −4.04
  FGA Fibrinogen alpha −2.61 -
  PLG Plasminogen −1.79 -
  PROC Protein C −1.39 −3.54
  SERPINA1 Antitrypsin −1.75 -
  SERPIND1 Heparin cofactor −2.00 −1.50
  THBS2 Thrombospondin 2 −1.17 -
Adipokines ADIPOQ Adiponectin 1.03 −1.48
  ANGPTL4 Angiopoietin-like 4 1.01 −1.58
  ATRN Attractin −1.12 −1.22
  CFD Adipsin 1.24 -
  LPL Lipoprotein lipase - −1.41
  NAMPT Visfatin - −1.20
  RARRES2 Chemerin −1.32 −1.54
  RBP4 Retinol binding protein 4 −2.33 −1.11
Lipogenesis DHCR7 7-Dehydrocholesterol reductase 1.11 -
  FADS2 Fatty acid desaturase 2 1.21 -
  FASN Fatty acid synthase 1.36 1.60
  G6PC Glucose-6-phosphatase, catalytic subunit 1.46 -
  scGH Growth hormone, chicken, short form 1.15 -
  HMGCR 3-Hydroxy-3-methylglutaryl-CoA reductase 1.09 -
  INSIG2 Insulin induced gene 2 1.74 -
  LCAT Lecithin-cholesterol acyltransferase 1.32 -
  MVD Mevalonate (diphospho) decarboxylase 1.20 -
  SCD Stearoyl-CoA desaturase 1.48 1.88
  SREBF1 Sterol regulatory element binding transcription factor 1 1.12 1.32
  THRSPA Thyroid hormone responsive spot 14 A - 1.64
  TXNIP Thioredoxin interacting protein - 1.80
Lipolysis ACAT1 Acetyl-CoA acetyltransferase 1 −3.18 -
  ADH1C Alcohol dehydrogenase 1C (class I), gamma polypeptide −1.81 -
  APOA1 Apolipoprotein A-I −1.16 -
  APP Amyloid beta (A4) precursor protein −1.15 -
  BCMO1 beta-carotene 15,15′-monooxygenase - −1.13
  BCO2 beta-carotene oxygenase 2 −1.15 −1.48
  CYP27A1 Cytochrome P450, family 27, subfamily A, polypeptide 1 −1.14 -
  CYP2E1 Cytochrome P450, family 2, subfamily E, polypeptide 1 −1.80 -
  EHHADH Enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase −1.09 -
  GAMT Guanidinoacetate N-methyltransferase −1.21 -
  HADHB Hydroxyacyl-CoA dehydrogenase (trifunctional protein) −1.10 -
  HSD17B4 Hydroxysteroid (17-beta) dehydrogenase 4 −1.92 -
  HSD17B6 Hydroxysteroid (17-beta) dehydrogenase 6 −1.19 -
  IRS1 Insulin receptor substrate 1 −1.59 -
  PDK4 pyruvate dehydrogenase kinase, isozyme 4 - −1.99
  PHYH Phytanoyl-CoA 2-hydroxylase −1.56 -
  SLC2A2 Facilitated glucose transporter 2 (GLUT2) −2.23 -
  SOD3 superoxide dismutase 3, extracellular −1.10 −1.20
  TP53 Tumor protein p53 −1.29 -
  UCP3 Uncoupling protein 3 (mitochondrial, proton carrier) −1.21 -
  1. *Fold-change (FC) represents the ratio of FL/LL transcript abundance averaged across six juvenile ages (1–11 wk). Prior candidate genes were identified as differentially expressed (DE) genes by previous microarray or qRT-PCR analysis of previous genetic, nutritional or hormonal perturbation studies. Pearson’s correlation coefficient (r) of expression ratios (FL/LL) of 15 select genes subjected to both microarray and qRT-PCR analyses indicates a significant (P≤0.01) correlation between the two methods (r = 0.64). The exclusion of two genes with the lowest microarray FC estimate (ANGPTL4 and ADIPOQ) greatly increases the Pearson correlation coefficient (r = 0.79) and the significance level (P≤0.001).