Expression profile, northern analysis and secondary structure prediction of an mRNA-associated small RNA in S. venezuelae . (A) Expression profile of sven2374, which shows high read levels for both its 5′ end and an internal 62 nucleotide region. Relative sequence reads at each nucleotide position are shown in red (long transcript library) and orange (sRNA-enriched library). (B) Predicted secondary structure of sven2374_sr1 (sr1: stable region 1). (C) Northern blot showing the temporal expression of sven2374_sr1 (time of RNA extraction is indicated in hours). 5S rRNA was probed as a control for RNA integrity and RNA loading levels.