Figure 8

A systemic RNAi effect in grain aphid observed by using the fluorescent labeled dsRNA added to the artificial diet. The fluorescent labelled dsRNA were added to the artificial diet at a final concentration of 7.5 ng/μl. Fifteen third instar grain aphids were inoculated. (A) 1-2 h later, the fluorescence signal mainly observed in the mouthpart. (B) and (C) 3 h to 5 h later, strong fluorescent signals was mainly centralized in the midgut. (D) In the following time period of artificial diet feeding (6-24 h period), the fluorescent signal began spread around the whole body tissues. From A to D, the left panel of each line was observed under stereo microscope (SM), the middle panel of each line was on bright field (BF) and right panel was on 559 nm (Cy3) under Confocal Fluorescent Microscopy FV1000.