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Table 3 Primer sets used for qRT-PCR analysis of the selected candidate genes upon aphid feeding on wheat plants and in vitro dsRNA synthesis of 16 candidates

From: Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants

Unigene no.

Forward primer

Reverse primer

Unigene_6994

TAATACGACTCACTATAGGG AGCTGAAAAGTGAAATGATGGTGGTC

TAATACGACTCACTATAGGG TTGCCAAACTGGAAAATCG

Unigene_ 8273

TAATACGACTCACTATAGGG AGTGCGTCAGATAGTCCGGCACC

TAATACGACTCACTATAGGG CACCATTCAAATCCGTTTCTTACAGC

Unigene_ 8279

TAATACGACTCACTATAGGG AGAAAGCAATGCTGTGGCAGAAAC

TAATACGACTCACTATAGGG GTGGTCGTTCTTTCAGTGATGG

Unigene_11975

TAATACGACTCACTATAGGG AGCGCTGTTGGTGCTGGTGGTGATG

TAATACGACTCACTATAGGG TCGTTGGACGGTTTGGTCGATGTCT

Unigene_21088

TAATACGACTCACTATAGGG AGGTTTAACGAATCGGACCATCAGGAA

TAATACGACTCACTATAGGG AAAATATGATCGCCTCAAGGGGACT

Unigene_21789

TAATACGACTCACTATAGGG AGTACCGTCCGCAAATGGTTGG

TAATACGACTCACTATAGGG TCCGAAGTCACATGTTCGCA

Unigene_23028

TAATACGACTCACTATAGGG AGGTTATTGTTGAACCCTTCTGACACG

TAATACGACTCACTATAGGG GAAGGATTAGACGATATTTTGGTG

Unigene_23042

TAATACGACTCACTATAGGG AGGTTCCATGACAAGCCGGATAC

TAATACGACTCACTATAGGG GACTCAAGTGCCTGGTGGGT

Unigene_23235

TAATACGACTCACTATAGGG AGGGTGCCCGACTCGGTTTTCTCC

TAATACGACTCACTATAGGG GCTGCCCAAGCCAACATCCAC

Unigene_26956

TAATACGACTCACTATAGGG AGCCGTGATTCTCCTGCGTCTGCT

TAATACGACTCACTATAGGG AGTCTTCGCCACCGCCGTTT

Unigene_27357

TAATACGACTCACTATAGGG AGATGGTTTTATGGTTGGCTTTAGTCCAG

TAATACGACTCACTATAGGG CCGCGTTCGCATTTCGTTTT

Unigene_27882

TAATACGACTCACTATAGGG AGAGGTATCACCCGCCGTACC

TAATACGACTCACTATAGGG GAACAGCTGGTTCTGCGAAA

Unigene_28447

TAATACGACTCACTATAGGG AGTGGCCCGTACGACACACCGA

TAATACGACTCACTATAGGG CGGACGATGTGCCGGGTGAC

Unigene_28469

TAATACGACTCACTATAGGG AGCAAATGAAACGCCGTATTTGATAA

TAATACGACTCACTATAGGG CGTGCCTACCGTATTCGACAAT

Unigene_28555

TAATACGACTCACTATAGGG AGGGGTTAGACTTGCCCGAAACTGATG

TAATACGACTCACTATAGGG TCCATGTTGGCAGTCCAACCGT

Unigene_29698

TAATACGACTCACTATAGGG AGCGTCGCCTTGGTGAGCCTTTAC

TAATACGACTCACTATAGGG CGGCGGACGGGTGAGTAATG

Actin

CCGAAAAGCTGTCATAATGAAGACC

GGTGAAACCTTGTCTACTGTTACATCTTG

GFP

TAATACGACTCACTATAGGG AGGCAGTGCTTCAGCCGCTACCC

TAATACGACTCACTATAGGG CCTTGATGCCGTTCTTCTGCTTGTC

  1. Note: T7 promoter sequences were tailed to each 5’ end of primers and underlined. The sequences without T7 promoter sequence tails were used for qRT-PCR analysis of the selected candidate genes upon aphid feeding on wheat plants.
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BMC Genomics

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