Skip to main content
Figure 3 | BMC Genomics

Figure 3

From: RNA-Seq effectively monitors gene expression in Eutrema salsugineum plants growing in an extreme natural habitat and in controlled growth cabinet conditions

Figure 3

Principal component analysis of global gene expression in nine Eutrema plants. Expression estimates for 27,016 Eutrema genes in nine plants were log-transformed (see Methods) and subjected to PCA using a covariance matrix. (A) Scree plot illustrating the proportion of variance accounted for by each principal component. Scores and loading biplots of genes and cDNA libraries (red vectors) along principal components are shown in panels B, C, and D. (B) PC1 vs. PC2: Genes were divided into four equal bins (Q1 to Q4) according to their median expression level across all libraries and shaded to visualize relative expression level. Least abundantly expressed genes are black and most abundantly expressed genes are shaded light grey. (C) PC1 vs. PC3: Genes were divided into bins and indexed for expression level as described for B. (D) PC2 vs. PC3: ● gene expression detected only in YF plants; “orange circle symbol” gene expression detected only in YC plants; “blue circle symbol” gene expression detected only in SC plants. Genes represented by grey points were expressed in multiple plant groups.

Back to article page