Differential expression patterns of KLFs in HESC and primary erythroid cells. (A) Gene expression profile of KLF1-17 in undifferentiated HESC and three primary erythroid cells (ESER, FLER, and PBER) examined using mRNA-seq analysis. Gene expression intensity was calculated by normalizing the read counts to reads per kilobase of the exon model per million mapped reads (RPKM) according to the gene length and total mapped reads. KLF2 and KLF14 were not detected and thus were not shown in this figure, whereas KLF10a and 10b represented two KLF10 isoforms. (B) Gene expression profile of KLF1, 3, 6, 9, 10, 11, 13, and 16 in undifferentiated HESC and three primary erythroid cells examined using quantitative real-time PCR. Transcript levels of KLFs were calculated in relation to that of 18S ribosomal RNA, and the expression levels of KLFs in ESER, FLER, and PBER were normalized to those in HESC. The error bars above each column indicate standard error of the mean (SEM) between triplicates. The Y-axis breaks at 3. Asterisks indicate that the differences between the levels of individual transcripts in erythroid cells (with Y values ranging from 1 to 3) and those in HESC were statistically significant by independent-samples t-test, ***p < 0.001, **p < 0.005. (C) Gene expression profile of KLF2, 4, 5, 7, 8, 12, 14, 15, and 17 in undifferentiated HESC and three primary erythroid cells examined using quantitative real-time PCR.