Figure 2

Post-transcriptional regulatory network of miRNAs up-modulated in T (primary tumor) vs N (normal colon mucosa) contrast. (A) The bipartite network represents DEMs up-modulated (FC > 3) in T vs N comparison (red triangles), supported target genes (circles) and their relations (gray dotted lines). Target DEGs in T vs N contrast are shown in blue, other genes in grey. The pink solid line outlines the experimentally validated miR-182/ENTPD5 relation. (B) Inverse correlation between miR-182 and ENTPD5 expression, according to qRT-PCR in 5 colon cancer cell lines and a pool of normal tissue. (C) Luciferase reporter assay of 3′UTR region of ENTPD5 and miR-182. Average relative light units (RLU) of biological replicates compared with control (HEK293T pMIR-ENTPD5), non-target RNA (HEK293T pMIR-ENTPD5 non-target RNA) and miR-182 over-expression (HEK293T pMIR-ENTPD5 miR-182). Data shown as means ± standard deviation (SD) of mean of three experiments performed in triplicate. * P < 0.05 vs N or control. **P < 0.01 vs N or control. nRQ: normalized Relative Quantity.