Figure 5

Localizations of histone modifications relative to the NL. (A) Representative immunofluorescence staining images of histone modifications (green) and lamin B (red) in C2C12 myoblast nuclei. Scale bars are 5 μm. (B) Radial intensity plots integrated over the entire contour of NL from representative cell nucleus images of histone modifications: H3K4me1 (n=6), H3K4me2 (n=6), H3K9Ac (n=7), H3K9me2 (n=4), H3K27me3 (n=6), H3K36me3 (n=8), and H3K79me2 (n=9). Distances from the NL (x-axis labels, in μm) switch from negative to positive values where locations to measure pixel intensities progress from outside the NL to inside the NL, as described previously [23]. H3K4me2 (black) and H3K9me2 (red) intensities were used in each plot to represent histone modifications located away from the NL and located towards the NL, respectively. Error bars represent standard deviations. Two-sample t-test results of the normalized immunostaining fluorescence intensities within 0 to 0.5 μm distance from the NL center are the following: H3K4me2 vs H3K9me2: p < 0.001, H3K4me2 vs H3K27me3: p < 0.01, H3K4me2 vs H3K4me1: p < 0.05, H3K4me2 vs H3K9Ac: p = 0.95, H3K4me2 vs H3K36me3: p < 0.05, H3K4me2 vs H3K79me2: p = 0.08, H3K9me2 vs H3K4me1: p < 0.01, H3K9me2 vs H3K27me3: p < 0.01.