FIE binding coincides with H3K27me3 peaks. (a) Overlap of FIE-HA and H3K27me3 ChIP-seq peaks. (b) Distribution of FIE-HA and H3K27me3 ChIP-seq signal relative to gene coding regions. (c) Size distribution of FIE-HA+H3K27me3 and H3K27me3 peaks. Peaks were placed in 200 bp size bins. (d) Verification of FIE-HA ChIP-seq peaks by ChIP-qPCR. Amplicons correspond to peaks present in both FIE-HA+H3K27me3 (1–10), FIE-HA only (41–50), H3K27me3 only (51–60) and neither dataset (61–70); values are normalized to the average signal of the untransformed control. Amplicons 61–70 are as used in Figure 2. (e) H3K27me3 ChIP-qPCR for genes present in FIE-HA+H3K27me3, FIE-HA only, H3K27me3 only and neither dataset; values are enrichment relative to input DNA. (n.d.; not determined). Dotted lines are average H3K27me3 abundance for H3K27me3 + FIE (does not include FLC as this was not associated with an H3K27me3 peak), FIE-HA only and H3K27me3 only regions. Averages are 1.15, 0.34 and 0.67; the averages for H3K27me3+FIE and H3K27me3 only are different at p<0.001 and FIE-only and H3K27m3-only are different at p<0.01 using a two-tailed t-test.