Comparative analysis of the complete genome sequence of E. faecium Aus0085. (A) Alignment of E. faecium Aus0085 NcoI optical map with an in silico-generated NcoI map of the final chromosome assembly, demonstrating the accuracy of the assembly. (B) Alignment of the chromosomes from E. faecium strains Aus0004, Aus0085 and TX16, generated using the Artemis Comparison Tool. The gray bands located at the top, middle and bottom represent the forward and reverse DNA strands for the chromosome sequences belonging to Aus0004, Aus0085 and TX16 respectively. The red lines correspond to regions of similarity between two chromosomes. The blue lines correspond to regions of similarity between two chromosomes in differing orientations. White regions are those that are unique to one strain and absent in the other. (C) Circular map of ST203 Aus0085 showing DNA-DNA comparisons against ST17 Aus0004 and ST18 TX16. Track identification, moving outwards, is as follows: G + C content, GC skew, E. faecium Aus0085, E. faecium Aus0004, E. faecium TX16, and finally the location of prophage regions, Tn1549 transposon and the esp-pathogenicity island.