Analysis of Tn1549. (A) Chromosomal location and alignment of Tn1549 from E. faecium isolates Aus0004 (nucleotide positions 2835430–2869240) and Aus0085 (nucleotide positions 723825–781312, reverse orientation). Compared to Tn1549 in Aus0004, the element in E. faecium Aus0085 is 23.7 kb longer. White regions correspond to the unique 23.7 kb sequence. The red regions correspond to matches between the two transposons. (B) Transposon insertion site comparisons showing the left-hand side and right-hand side flanking regions and donor (coupling) sequences.