Figure 5

Nile red staining of cryosections from T. castaneum late larvae injected with dsRNA specific for TcVer and TcABCG-4C. To detect neutral lipids such as triglycerides, cryosections (transverse, 20 μm) prepared from the abdomen of late larvae were stained with Nile red. (A) dsRNA specific for TcVer was injected into penultimate larvae. The upper right panel shows a strong fluorescence labeling of fat body cells, fat droplets and cuticle (arrow) at low magnification; left panel, bright field micrograph. The lower panels show detail views of the cuticle in bright field (left) and fluorescence imaging (right). Nile red stains cuticular lipids of the epicuticle. The thickness of the cuticle is marked with a bracket. (B) dsRNA specific for TcABCG-4C was injected into penultimate larvae. The right panel shows only very weak fluorescence labeling of fat body cells and fat droplets, and no staining of the cuticle (arrow); left panel, bright field micrograph. The lower panels show detail views of the cuticle in bright field (left) and fluorescence imaging (right). Nile red did not stain the epicuticle.