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Table 1 Summary of SNP screening results for the Brassica napus BAC library

From: Screening of a Brassica napus bacterial artificial chromosome library using highly parallel single nucleotide polymorphism assays

Gene(s)

Number of known BACs

Number of confirmed BACs (% of known BACs)

Number of putative BACs

Number of confirmed BACs (% of putative BACs)

Assay

Gene(s)

Number of known BACs

Number of confirmed BACs (% of known BACs)

Number of putative BACs

Number of confirmed BACs (% of putative BACs)

BnaA.PKp3.a

10

10 (100%)

14

10 (71.4%)

#52

     

BnaA.PKp3.a

10

10 (100%)

19

10 (52.6%)

#51

BnaC.PKp3.a

15

12 (80%)

38

12 (31.6%)

BnaA.PKp3.a

10

10 (100%)

17

10 (58.8%)

#47

BnaC.PKp3.a

15

13 (86.7%)

35

13 (37.1%)

BnaA.KAS III.a

0

0

0

0

#7

BnaC.KAS III.a

4

4 (100%)

5

4 (80%)

BnaA.KAS III.a

0

0

0

0

#9

BnaC.KAS III.a

4

4 (100%)

5

4 (80%)

     

#2

BnaC.DGAT1.a

15

13 (86.7%)

39

13 (33.3%)

BnaA.DGAT1.a

2

1 (50%)

1

1 (100%)

#4

BnaC.DGAT1.a

16

14 (87.5%)

30

14 (46.7%)

BnaA.DGAT1.a

2

1 (50%)

1

1 (100%)

#5

BnaC.DGAT1.a

16

15 (93.8%)

33

15 (45.5%)

BnaA.DGAT1.b

16

13 (81.3%)

33

13 (39.4%)

#9A

BnaC.DGAT1.b

1

0

0

0

BnaA.DGAT1.b

16

13 (81.3%)

38

13 (34.2%)

#10A

BnaC.DGAT1.b

1

1 (100%)

1

1 (100%)

     

#13A

BnaC.DGAT1.c

10

9 (90%)

10

9 (90%)

     

#14A

BnaC.DGAT1.c

10

7 (70%)

7

7 (100%)

BnaA.WRI1.a

5

4 (80%)

5

4 (80%)

#32

BnaC.WRI1.a

9

8 (88.9%)

11

8 (72.7%)

BnaA.WRI1.a

5

4 (80%)

6

4 (66.7%)

#37

BnaC.WRI1.a

9

8 (88.9%)

11

8 (72.7%)

BnaA.WRI1.b

14

13 (92.9%)

36

13 (36.1)

#28

BnaC.WRI1.b

11

11 (100%)

52

11 (21.2%)

BnaA.WRI1.b

14

14 (100%)

534

14 (2.6%)

#30

BnaC.WRI1.b

11

2 (18.2%)

13

2 (15.4%)

BnaA.PKp1.a

9

0

0

0

#7A

     

BnaA.PKp1.a

9

1 (11.1%)

2

1 (50%)

#57

BnaC.PKp1.a

7

7 (100%)

10

7 (70%)

BnaA.PKp1.a

9

0

0

0

#60

BnaC.PKp1.a

7

5 (71.4%)

16

5 (31.3%)

BnaA.PKp1.a

9

2 (22.2%)

2

2 (100%)

#3A

BnaC.PKp1.a

7

4 (57.1%)

4

4 (100%)

BnaA.PKp1.a

9

3 (33.3%)

3

3 (100%)

#8A

BnaC.PKp1.a

7

2 (28.6%)

2

2 (100%)

     

#58

BnaC.PKp1.a

7

3 (42.9%)

3

3 (100%)

     

#55

BnaC.PKp1.b

18

11 (61.1%)

45

11 (24.4%)

     

#56

BnaC.PKp1.b

18

13 (72.2%)

50

13 (26%)

BnaA.FUS3.a

18

17 (94.4%)

108

17 (15.7%)

#14

BnaC.FUS3.a

20

13 (65%)

77

13 (16.9%)

BnaA/C.FUS3.a

38

36 (94.7%)

2492

36 (1.4%)

#10

     

BnaA/C.FUS3.a

38

37 (97.4%)

3047

37 (1.2%)

#12

     

BnaA/C.FUS3.a

38

36 (94.7%)

2249

36 (1.6%)

#18

     

BnaA/C.FUS3.a/b

62

58 (93.5%)

12114

58 (0.5%)

#25

     

BnaA.FUS3.b

8

5 (62.5%)

10

5 (50%)

#20

BnaC.FUS3.b

16

16 (100%)

38

16 (42.1%)

BnaA.FUS3.b

8

8 (100%)

12

8 (66.7%)

#21

BnaC.FUS3.b

16

14 (87.5%)

33

14 (42.4%)

BnaA.FUS3.b

8

5 (62.5%)

9

5 (55.6%)

#22

BnaC.FUS3.b

16

16 (100%)

67

16 (23.9%)

BnaA.BCCP2.a

12

11 (91.7%)

19

11 (57.9%)

#45

     

BnaA.BCCP2.a

12

10 (83.3%)

17

10 (58.9%)

#70

     
     

#43

BnaC.BCCP2.a

13

10 (76.9%)

36

10 (27.8%)

     

#44

BnaC.BCCP2.a

13

11 (84.6%)

39

11 (28.2%)

BnaA.BCCP2.b

9

9 (100%)

10

9 (90%)

#2A

     

BnaA/C.BCCP2.b

21

20 (95.2%)

337

20 (5.9%)

#65

     
     

#41

BnaC.BCCP2.b

12

11 (91.7%)

99

11 (11.1%)

  1. In one of the BAC clones which contained locus BnaC.DGAT1.a only a segment of the gene was present, therefore identification of this clone was only possible with assays #4 and #5 but not with #2.
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