Figure 3
From: RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae
Examples of cultures of Verticillium dahliae that were used to determine relative gene expression by real-time quantitative PCR. A) A darkly pigmented 12 day culture of V. dahliae in which microsclerotia have formed (MS +). B) A 12 day culture of V. dahliae in which MS have not formed (NoMS). C) A 7 day culture of V. dahliae in which MS formation has occurred, but at an intermediate level relative to cultures A and D (note the slight pigmentation in C). Cultures A-C were maintained at 25°C. D) A culture stored for approximately 6 months at 4°C after an initial incubation period of 4 days at 25°C. The gridded circular nitrocellulose membranes, which allowed easy harvest of the fungal MS and mycelia, are visible in A – C, but obscured in D.