Differential splicing of stk16 under cold stress. (A) Read coverage at stk16 locus. The top panel shows the read coverage of each sample at sk16 locus and the bottom panel indicates the structure of stk16 transcripts. The red arrowheads represent relative positions of primers for RT-PCR. The dashed red box indicates the unchanged coverage of exon 2 under cold stress. (B) RT-PCR analysis to reveal the cold-specificity of stk16-J2. Stk16-J2-F and stk16-J2-R primers were displayed in (A). (C) The relative abundance of stk16 isoforms under cold stress. (D) Partial peptide sequence alignment of Stk16-J1 and Stk16-J2. The identical amino acids are shown in yellow.