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Table 3 Confirmation of gene expression base on mRNA-Seq assay using qPCR

From: Analysis of Phakopsora pachyrhizi transcript abundance in critical pathways at four time-points during infection of a susceptible soybean cultivar using deep sequencing

  Time-points
Gene description Technique 15 sai 7 hai 48 hai 10 dai
Alpha-tubulin qPCRa 11 30 34 1,848
mRNA-Seqb NAc NA NA 1.78
NADH dehydrogenase subunit f qPCR 293,119 214,031 225,034 548,514
mRNA-Seq 1.25 0.15 NA NA
Ribulose-1,5-bisphosphate carboxylase oxygenase large subunit qPCR 813,754 730,477 708,526 1,794,680
mRNA-Seq 0.21 NA NA 1.2
Pectin methylesterase qPCR 6,726 8,281 5,649 6,488
mRNA-Seq NA 0.084 NA NA
Maturase-related qPCR 20,598 28,055 15,242 33,772
mRNA-Seq NA NA 0.068 NA
Serine palmitoytransferase qPCR 0 3 6 609
mRNA-Seq NA NA NA 0.034
60S ribosomal protein L18 qPCR 0 0 11 1,238
  mRNA-Seq NA NA NA 2.87
  1. aValues used to describe qPCR results are absolute number of specific transcript normalized against the absolute number of α-tubulin transcript in the initial sample.
  2. bValues used to describe mRNA-Seq results are normalized coverage explain in the Methods section.
  3. cNot applicable since the transcript was not sequenced.