Analysis of Phakopsora pachyrhizi transcript abundance in critical pathways at four time-points during infection of a susceptible soybean cultivar using deep sequencing

Table 3 Confirmation of gene expression base on mRNA-Seq assay using qPCR

		Time-points
Gene description	Technique	15 sai	7 hai	48 hai	10 dai
Alpha-tubulin	qPCR^a	11	30	34	1,848
Alpha-tubulin	mRNA-Seq^b	NA^c	NA	NA	1.78
NADH dehydrogenase subunit f	qPCR	293,119	214,031	225,034	548,514
NADH dehydrogenase subunit f	mRNA-Seq	1.25	0.15	NA	NA
Ribulose-1,5-bisphosphate carboxylase oxygenase large subunit	qPCR	813,754	730,477	708,526	1,794,680
	mRNA-Seq	0.21	NA	NA	1.2
Pectin methylesterase	qPCR	6,726	8,281	5,649	6,488
Pectin methylesterase	mRNA-Seq	NA	0.084	NA	NA
Maturase-related	qPCR	20,598	28,055	15,242	33,772
Maturase-related	mRNA-Seq	NA	NA	0.068	NA
Serine palmitoytransferase	qPCR	0	3	6	609
Serine palmitoytransferase	mRNA-Seq	NA	NA	NA	0.034
60S ribosomal protein L18	qPCR	0	0	11	1,238
	mRNA-Seq	NA	NA	NA	2.87

^aValues used to describe qPCR results are absolute number of specific transcript normalized against the absolute number of α-tubulin transcript in the initial sample.
^bValues used to describe mRNA-Seq results are normalized coverage explain in the Methods section.
^cNot applicable since the transcript was not sequenced.

ISSN: 1471-2164