Analysis of significantly extended protein families. A. Gene tree of proteins with the Pfam domain PF00690 (P-type ATPase transmembrane transporters). The tree was constructed with the PhyML 3.0 software. Chi2-based approximate Likelihood-Ratio Test branch supports are shown for the major groups. Abbreviations of fungal species from which the proteins originate: Ab, Agaricus bisporus; An, Aspergillus nidulans; Ca, Candida albicans; Cc, Coprinopsis cinerea; Cn, Cryptococcus neoformans var. grubii; Mg, Malassezia globosa; Nc, Neurospora crassa; Pg, Puccinia graminis; Ro, Rhizopus oryzae; Rg, Rhodotorula graminis; Sc, Saccharomyces cerevisiae; Um, Ustilago maydis; Wi, Wallemia ichthyophaga; Ws, Wallemia sebi. The groups are labelled with the cations that are transported by the given group of ATPases, and the names of the S. cerevisiae proteins in that group (in brackets). B. Top: Proportion of acidic amino acids in hydrophobins from various fungal species. Box chart of quartiles shows the molar pecentages of acidic amino acids in individual hydrophobin proteins, with minima and maxima shown by the whiskers. Ustilago maydis contains only one hydrophobin of expected length. Bottom: The graphical representation of multiple sequence alignment of all hydrophobins from a given species shows the conservation of individual positions and amino acids. Black, acidic amino acids; light grey lined with black, cysteine residues; grey, all other residues.