Location of RNA processing sites in the 5′ UTR of pnp mRNA. (A) shows the gRNA-seq and dRNA-seq data, while (B) shows the location of the processing sites relative to the secondary structure of the 5′ UTR as predicted using MFold . The labelling of (A) is as Figure 2. The RNA-seq data for pnp is depicted by red negative bars as this gene is encoded on the reverse strand. In (B) the nucleotide positions are numbered relative to the TSS, while the sites of processing are numbered relative to the genome.