Figure 6

An oncogenic role for KLF5. (A) By aCGH, KLF5 is found focally amplified in HPAC pancreatic cancer cells. Data plotted as described in Figure 4A legend. (B) Screen profiles of KLF5 shRNAs. Above left, heatmap depicts shRNA depletion/enrichment (blue/red) for each KLF5-targeting shRNA across the cell line panel; R and q values are indicated (see Figure 3 legend). For select cell lines, Western-blot verification of KLF5 on-target knockdown was done by individual shRNA transfection (above right) or siRNA transfection (below right). Effect of knockdown on cell growth/viability was independently verified by siRNA transfection (below left); P-values by Student’s t-test. (C) KLF5 on-target siRNA knockdown also verified by Q-RT-PCR. (D) Relative transcript levels of KLF5, from microarray data [6], across 20 pancreatic cell lines; red and blue intensity reflects increased and decreased expression, respectively. (E) Plot of KLF5 relative transcript levels (x-axis) vs. average KLF5-targeted shRNA depletion/enrichment (y-axis). Note that most cell lines expressing KLF5 transcript are KLF5-dependent, except for Panc1 cells in which KLF5 transcript is barely detectable. (F) Knockdown of KLF5 (verified by Western, above) results in reduced growth/viability of Panc5.04 cells, heterozygous for the KLF5 (H389N) mutation. P-value by Student’s t-test (KLF5 siRNA vs. non-targeting control siRNA).