Table 2 Summary of validation for target knockdown and growth phenotype
From: Integrative genomic and functional profiling of the pancreatic cancer genome
Gene | shRNA knockdown | shRNA knockdown | shRNA knockdown | siRNA knockdown | siRNA knockdown | siRNA knockdown |
|---|---|---|---|---|---|---|
| Â | validated by RT-PCR | validated by | validated by cancer | validated by | validated by | growth phenotype |
| Â | Â | western blot | genome anatomy | RT-PCR | western blot | validated |
| Â | Â | Â | Project[25] | Â | Â | Â |
KRAS | Â | X [26] | Â | X | X | X |
SMURF1 | Â | X | Â | Â | X | X |
CDKN2A | Â | Â | X | Â | Â | Â |
CCNE1 | Â | Â | X | Â | Â | Â |
NUP153 | X | Â | Â | Â | X | X |
KLF5 | Â | X | Â | X | X | X |