Reads | Number of reads | Bases |
---|
BR | 1,182,916 | 425,618,400 |
BS | 1,177,524 | 426,564,503 |
Aligned* | 2,200,571 | 785,888,510 |
(93.2%) | (92.2%) |
Assembled* | 1,981,006 | 54,877,004 |
Partially aligned* | 219,291 | - |
Singletons* | 133,795 | - |
Repeats* | 471 | - |
Outliers* | 24,280 | - |
- *Definitions of these Newbler de novo assembly terms can be found in Additional file 5: Text S1.
- Sequence libraries of one resistant bulk (BR) and one susceptible bulk (BS) each containing pooled cDNA of 16 individual F2 plants were generated to perform the 454 sequencing.