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Table 1 Read statistics of 454 cDNA sequences after quality filtering

From: BSTA: a targeted approach combines bulked segregant analysis with next- generation sequencing and de novo transcriptome assembly for SNP discovery in sunflower

Reads Number of reads Bases
BR 1,182,916 425,618,400
BS 1,177,524 426,564,503
Aligned* 2,200,571 785,888,510
(93.2%) (92.2%)
Assembled* 1,981,006 54,877,004
Partially aligned* 219,291 -
Singletons* 133,795 -
Repeats* 471 -
Outliers* 24,280 -
  1. *Definitions of these Newbler de novo assembly terms can be found in Additional file 5: Text S1.
  2. Sequence libraries of one resistant bulk (BR) and one susceptible bulk (BS) each containing pooled cDNA of 16 individual F2 plants were generated to perform the 454 sequencing.