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Figure 1 | BMC Genomics

Figure 1

From: Deep sequencing uncovers commonality in small RNA profiles between transgene-induced and naturally occurring RNA silencing of chalcone synthase-A gene in petunia

Figure 1

Detection of CHS-A mRNA and siRNAs in J-type and Red Star flowers. (a) Flower phenotypes of J-type (left) and Red Star (right) plants. (b) Steady state mRNA levels of the CHS-A gene in the white and pigmented petal tissues examined by RT-PCR. J-p, pigmented portions of petals in J-type; J-w, white portions of petals in J-type; R-p, pigmented portions of petals in Red Star; R-w, white portions of petals in Red Star. Transcripts of α-tubulin gene were amplified as a positive control. A reaction mixture without reverse transcriptase was used as a control to confirm that no amplification occurred from genomic DNA contamination of the RNA sample (RT-). (c) Detection of CHS-A siRNAs by Northern blot analysis. Same tissues were used as in the RT-PCR. Hybridization signals obtained with two exposure durations are shown. Ethidium-bromide-stained tRNA and 5S rRNA bands are shown below the panels to show that an equal amount of the small RNA fraction was loaded.

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BMC Genomics

ISSN: 1471-2164

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