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Table 1 Tissue and temperature combinations used in constructing the reference transcriptome of P. borchgrevinki

From: Model of gene expression in extreme cold - reference transcriptome for the high-Antarctic cryopelagic notothenioid fish Pagothenia borchgrevinki

Treatment

Liver

Brain

White muscle

Spleen

Head kidney

Gill

Lens

Native

 

Control, -1 to −1.9°C

yes

yes

yes

yes

yes

yes

yes

Warm Acclimation

 

2 days, 4°C

yes

yes

yes

-

yes

yes

-

4 days, 4°C

yes

yes

yes

yes

yes

yes

-

7 days, 4°C

yes

yes

yes

yes

-

yes

-

2 months, 4°C

yes

yes

-

yes

yes

-

-

Acute Heat Stress

 

Post-CTMax

yes

yes

yes

yes

yes

-

-

  1. Overview of the tissues included in the reference transcriptome. Total RNA was extracted from one specimen for each tissue/treatment combination and pooled at equal concentrations. P. borchgrevinki reference transcriptome was then produced by sequencing this pool on a Roche 454 GS FLX+ Titanium sequencer.