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Table 1 Phenotype and markers expression levels in WJ-MSC at 4 th and 12 th passage

From: Gene expression modifications in Wharton’s Jelly mesenchymal stem cells promoted by prolonged in vitro culturing

Antigens Phenotype 4thpassage 12thpassage p <
MFI ratio ± S.D.
CD13 + 11.6 ± 0.8 7.7 ± 0.8 0.002
CD14 - 1.3 ± 0.1 1.1 ± 0.1 0.222
CD29 +++ 154.6 ± 7.3 141.9 ± 2.4 0.036
CD34 - 1.3 ± 0.1 1.1 ± 0.1 0.210
CD44 +++ 173.8 ± 20.1 82.0 ± 8.7 0.009
CD45 - 1.1 ± 0.2 1.2 ± 0.2 0.308
CD73 ++ 49.5 ± 2.7 19.9 ± 1.1 0.001
CD90 ++ 78.4 ± 2.6 81.0 ± 3.2 0.226
CD105 + 8.7 ± 0.7 7.9 ± 0.5 0.218
CD117 - 1.8 ± 0.2 1.5 ± 0.2 0.152
CD133 - 1.5 ± 0.1 1.2 ± 0.1 0.094
CD144 - 1.2 ± 0.1 1.3 ± 0.2 0.320
CD146 + 10.5 ± 0.6 11.3 ± 0.6 0.230
CD166 + 14.4 ± 0.9 6.8 ± 1.2 0.014
CD326 - 1. 1 ± 0.1 1.1 ± 0.1 0.420
HLA-ABC + 24.0 ± 0.4 24.9 ± 0.6 0.023
HLA-DR - 1.3 ±0.1 1.1 ± 0.1 0.341
  1. – negative expression; + moderate expression; ++ positive; +++ high expression; MFI Ratio is the average of five different biological samples ± standard deviation; Bold values represent MFI Ratio With p < 0.01; Cut-off positivity MFI Ratio > 2.