Skip to main content

Table 1 Phenotype and markers expression levels in WJ-MSC at 4 th and 12 th passage

From: Gene expression modifications in Wharton’s Jelly mesenchymal stem cells promoted by prolonged in vitro culturing

Antigens

Phenotype

4thpassage

12thpassage

p <

MFI ratio ± S.D.

CD13

+

11.6 ± 0.8

7.7 ± 0.8

0.002

CD14

-

1.3 ± 0.1

1.1 ± 0.1

0.222

CD29

+++

154.6 ± 7.3

141.9 ± 2.4

0.036

CD34

-

1.3 ± 0.1

1.1 ± 0.1

0.210

CD44

+++

173.8 ± 20.1

82.0 ± 8.7

0.009

CD45

-

1.1 ± 0.2

1.2 ± 0.2

0.308

CD73

++

49.5 ± 2.7

19.9 ± 1.1

0.001

CD90

++

78.4 ± 2.6

81.0 ± 3.2

0.226

CD105

+

8.7 ± 0.7

7.9 ± 0.5

0.218

CD117

-

1.8 ± 0.2

1.5 ± 0.2

0.152

CD133

-

1.5 ± 0.1

1.2 ± 0.1

0.094

CD144

-

1.2 ± 0.1

1.3 ± 0.2

0.320

CD146

+

10.5 ± 0.6

11.3 ± 0.6

0.230

CD166

+

14.4 ± 0.9

6.8 ± 1.2

0.014

CD326

-

1. 1 ± 0.1

1.1 ± 0.1

0.420

HLA-ABC

+

24.0 ± 0.4

24.9 ± 0.6

0.023

HLA-DR

-

1.3 ±0.1

1.1 ± 0.1

0.341

  1. – negative expression; + moderate expression; ++ positive; +++ high expression; MFI Ratio is the average of five different biological samples ± standard deviation; Bold values represent MFI Ratio With p < 0.01; Cut-off positivity MFI Ratio > 2.