Figure 6

qRT-PCR results showing the relative expression levels of the A. ipsilon pheromone biosynthesis related genes between the pheromone gland (PG) and the body (BO). The putative enzyme names are indicated as gene abbreviations followed by Genbank accession numbers. ACC Acetyl-CoA carboxylase, FAS Fatty acid synthase, DES Desaturase, FAR Fatty acyl reductase, AOX alcohol oxidase, AR Aldehyde reductase, ATF Acetyltransferase. The internal control β-actin and ribosomal protein S3 were used to normalize transcript levels in each sample. This figure was presented using β-actin as reference gene to normalize the target gene expression and correct sample-to-sample variation; similar results were also obtained with ribosomal protein S3 as reference gene. The standard error is represented by the error bar, and the different letters (a, b) above each bar denote significant differences (p >0.05).