Figure 2

Comparison of SeqA ChIP-Chip data of Sánchez-Romero et al. [11] (red) and Waldminghaus et al. [10] (green). Both studies used E. coli dnaC2 mutant cells synchronized regarding DNA replication 5 or 6 minutes after initiation and the same antibody for the ChIP reaction. While Sánchez-Romero and colleagues used the original ChIP-Chip protocol, Waldminghaus and colleagues used the modified protocol. While both signal patterns correspond to the GATC density (blue) as expected for SeqA, the dynamic range varies between 5.1 for the Sánchez-Romero et al. experiment and 98.6 for the Waldminghaus et al. experiment. Grey dots show corresponding peaks to a GATC density of ≥ 5 (Moving window of 500 bp; step size 100 bp).