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Table 4 Fitness analysis of resistance loci, tc_ rph1 and tc_ rph2 over 18 generations in the absence of phosphine selection

From: Rapid genome wide mapping of phosphine resistance loci by a simple regional averaging analysis in the red flour beetle, Tribolium castaneum

Generations

Insects tested

Genotypes

Allelic frequency

Chi-square analysis on genotypes†

rr

rs

ss

p allele

q allele

χ2(df = 1)

P value

tc_ rph1

F2

94

28

41

27

0.51

0.49

2.15

0.143

F5

94

34

43

17

0.59

0.41

6.83*

0.009

F10

96

38

45

13

0.63

0.37

13.4**

<0.001

F15

95

35

46

14

0.61

0.39

9.38*

0.002

F20

96

38

48

8

0.65

0.34

18.8**

<0.001

tc_ rph2

F2

94

20

52

22

0.49

0.51

1.15

0.283

F5

96

5

54

37

0.33

0.67

22.8**

<0.001

  F10

92

7

38

45

0.29

0.71

33.6**

<0.001

F15

96

0

28

64

0.15

0.85

99.0**

<0.001

F20

96

0

36

58

0.19

0.81

75.2**

<0.001

  1. * Significant (P<0.01); ** Significant (P<0.001).
  2. † Significance values are calculated from deviation from Hardy-Weinberg equilibrium compared to expected F2 (1:2:1) ratio.