Figure 6

Confirmation of the targets of miR399, miR2111, and miR159e-3p by RLM-5’ RACE. Degradome sequencing analysis indicating a cleavage site at position 1050 from the 5’ end of the transcript of Glyma13g25716.1 is targeted by miR159e-3p (A). Target prediction of miR2111 with WMD3 (http://www.weigelworld.com) showing Glyma19g25770 and Glyma16g06160 as putative targets of miR2111 (B). Cleavage sites from the 5’ end for Glyma13g31290 (PHO2-1) by miR399 (C), GmPT5 by miR399 (D), Glyma13g25716 by miR159e-3p (E), and Glyma16g06160 by miR2111 (F). The arrow indicates the cleavage position in the transcript of target genes, and the number above the arrow shows the sequenced clone numbers of PCR products.