Genome-wide identification of soybean microRNAs and their targets reveals their organ-specificity and responses to phosphate starvation

Table 2 Conserved and less-conserved miRNAs families detected in four small RNA libraries from soybean

Family	Size (nt)	Site (5’ to 3’)	Homology in plant species
		1st	19th	A.thaliana	O.sativa	Z.mays	M.truncatula	P.trichocarpa
Conserved ^a
MIR156	20-21, 23	U (82%)	A (59%)	+	+	+	+	+
MIR159	21	U (100%)	C (100%)	+	+	+	+	+
MIR160	21	G (100%)	A (100%)	+	+	+	+	+
MIR162_1	21	U (100%)	C (100%)	+	−	−	+	+
MIR164	21	U (100%)	G (100%)	+	+	+	+	+
MIR166	20-22	U (80%)	C (50%)	+	+	+	+	+
MIR167_1	21-22	U (100%)	C (100%)	+	+	+	+	+
MIR168	20-21	C (67%)	A (67%)	+	+	+	+	+
MIR169_2	21	A (67%)	C (100%)	+	+	+	+	+
MIR172	21	U/A/G (33%)	C (67%)	+	+	+	+	+
MIR390	21	A (100%)	G (75%)	+	+	+	+	+
MIR396	21	G (60%)	A (60%)	+	+	+	+	+
MIR397	21	U (100%)	A (100%)	+	+	+	−	+
MIR399	21	U (100%)	C (100%)	+	+	−	+	+
MIR2118	22	U (100%)	C (100%)	−	+	+	+	+
MIR408	20-21	C (67%)	A (50%)	+	+	+	+	+
MIR482	21-22	U (56%)	A (33%)	−	−	−	−	+
Less-conserved ^b
MIR1507	22	U (100%)	C (100%)	−	−	−	+	−
MIR1508	21-22	U/C (50%)	U/G (50%)	−	−	−	−	−
MIR1509	21-22	U (100%)	G (100%)	−	−	−	+	−
MIR1510	21-22	A (75%)	U (50%)	−	−	−	+	−
MIR2109	21	U (100%)	U (100%)	−	−	−	−	−
MIR3522	22	U (100%)	U (100%)	−	−	−	−	−

The miRNAs in four soybean small RNA libraries were classified into conserved and less-conserved miRNAs based on the data in miRBase (http://www.mirbase.org).
^aFor conserved miRNAs, the counterpart of which can be found in other reference plants apart from legume.
^bFor less-conserved miRNAs, the homology of which can only be found in legume.
⁺indicates the existence of miRNA in species, and - shows no homology in plant species.
A, adenosine; C, cytosine; G, guanosine; nt, nucleotide; U, uridine.
A.thaliana, Arabidopsis thaliana; O.sativa, Oryza sativa; Z.mays, Zea mays; M.truncatula, Medicao truncatula; P.P.trichocarpa, Poplus trichocarpa.

ISSN: 1471-2164