Genome-wide identification of soybean microRNAs and their targets reveals their organ-specificity and responses to phosphate starvation

Table 5 Novel soybean miRNAs in four small RNA libraries

miRNA	Sequence (5’ to 3’)	Size (nt)	Ch	Start:End (+/ −)	Arm	TPM in HPL	TPM in LPL	TPM in HPR	TPM in LPR	Registered in
										miRBase or not
miRnov_1a	AAAGCCATGACTTACACACGC	21	17	1401437:1401518: −	5	160	173	271	249	No
miRnov_1b	AAAGCCATGACTTACACACGC	21	20	223678:223767: −	5	163	179	281	259	NO
miRnov_2	ATTGGGACAATACTTTAGATA	21	18	52797184:52797492:+	3	0.01	0.01	0.01	153	NO
miRnov_3	GGAGATGGGAGGGTCGGTAAAG	21	20	35349749:35349874:+	5	419	371	351	595	NO
miRnov_4	ATATGGACGAAGAGATAGGTAA	21	20	40357028:40357130:+	5	115	120	249	187	NO
miRnov_5a	CAGGGGAACAGGCAGAGCATG	21	2	837420:837549:+	5	394	307	86	15	NO
miRnov_5b	CAGGGGAACAGGCAGAGCATG	21	10	36557001:36557133: −	5	394	307	86	15	NO
miRnov_6	AGAGGTGTATGGAGTGAGAGA	21	13	25849778:25849881:+	5	256	122	96	98	NO
miRnov_7	AGCTGCTCATCTGTTCTCAGG	21	15	4318784:4318876:+	3	48	137	0.01	0.01	NO
miRnov_8	GCTCACTACTCTTTCTGTCGGTT	21	17	37759439:37759543:+	3	609	615	18	24	NO
miRnov_9	TCAATCCTGGAAGAACCGGCG	21	13	35514890:35515064:+	3	44	35	106	51	NO
miRnov_10	AGGAAGCTAAGACGGAACTTA	21	14	49069088:49069204:+	5	44	0.01	110	87	NO

Soybean novel miRNAs were identified based on Blast analysis of mature miRNA sequences. For novel miRNAs, which were not previously in miRBase and not assigned into any family were denoted by miRnov (soybean-specific).
Ch, chromosome; HPL, leaf+Pi; HPR, root+Pi; LPL, leaf-Pi; LPR, root-Pi; nt, nucleotide; TPM, transcript per million reads; +, sense strand; −, antisense strand.

ISSN: 1471-2164