Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)

Table 3 Follow-up qPCR of gene expression changes on selected genes

Gene name	Array (N = 4)	p-value	qPCR (N = 8)	p-value
Gene name	Log2 change over control	p-value	Log2 change over control	p-value
17-beta hydroxysteroid dehydrogenase 3	3.16	0.019	1.831	0.028
Activating transcription factor 1	3.05	0.032	1.860	0.188
Acetyl-CoA acyltransferase 2	2.40	0.031	0.951	0.405
Androgen receptor beta	−1.32	0.033	−0.486	0.505
zona pellucida glycoprotein 2	−6.487	0.0001	−7.6603	<0.001

ISSN: 1471-2164