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Figure 1 | BMC Genomics

Figure 1

From: RIP-seq of BmAgo2-associated small RNAs reveal various types of small non-coding RNAs in the silkworm, Bombyx mori

Figure 1

RIP for the isolation of BmAgo2-associated RNAs from BmN cells. (A) Co-Immunoprecipitation of HIS-BmAgo2 using HIS monoclonal antibody. IP was validated by Western blotting using a HIS monoclonal antibody. BmAgo2 was successfully expressed in BmN cells infected with recombinant BmNPV virus (lane 1) and was pulled down when the HIS antibody was used (lane 3). Mouse IgG1 was used as a negative control and indeed revealed no IP of BmAgo2 (lane 2). (B) Co-immunoprecipitated RNAs analyzed by PAGE. Compared to the total RNAs of the BmN cells (lane 3), the HIS monoclonal antibody pull-down RNAs showed an obvious RNA smear ranging from 20 bp to 50 bp (lane 1). The negative control mouse IgG1 did not immunoprecipitate visible RNA amounts (lane 2). Lane M shows a DNA size marker. Asterisks indicate the visible bands for the ~20nt, ~27nt and ~33nt small RNA species, respectively.

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