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Table 1 Yields, amplification, and rates of strand-invasion artifacts in libraries made with the RNA- (R3), DNA- (D3), or LNA-based (L3) TS oligonucleotides

From: Comparison of RNA- or LNA-hybrid oligonucleotides in template-switching reactions for high-speed sequencing library preparation

TS oligonucleotide

qPCR yield (Ct value)

Semisupressive PCR cycles

Strand invasion (%)

R3

20.4

23

9.1 ± 0.7

D3

23.6

23

73.6 ± 3.4

L1

22.8

23

48.6 ± 6.9

L2

26.2

28

38.6 ± 8.1

L3

27.5

28

35.9 ± 4.2