From: Comparison of RNA- or LNA-hybrid oligonucleotides in template-switching reactions for high-speed sequencing library preparation
TS oligonucleotide
qPCR yield (Ct value)
Semisupressive PCR cycles
Strand invasion (%)
R3
20.4
23
9.1 ± 0.7
D3
23.6
73.6 ± 3.4
L1
22.8
48.6 ± 6.9
L2
26.2
28
38.6 ± 8.1
L3
27.5
35.9 ± 4.2