Skip to main content

Table 1 Yields, amplification, and rates of strand-invasion artifacts in libraries made with the RNA- (R3), DNA- (D3), or LNA-based (L3) TS oligonucleotides

From: Comparison of RNA- or LNA-hybrid oligonucleotides in template-switching reactions for high-speed sequencing library preparation

TS oligonucleotide qPCR yield (Ct value) Semisupressive PCR cycles Strand invasion (%)
R3 20.4 23 9.1 ± 0.7
D3 23.6 23 73.6 ± 3.4
L1 22.8 23 48.6 ± 6.9
L2 26.2 28 38.6 ± 8.1
L3 27.5 28 35.9 ± 4.2