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Table 1 P. micrantha chloroplast sequencing data statistics

From: An evaluation of the PacBio RS platform for sequencing and de novo assembly of a chloroplast genome

 

PacBio RS

Illumina HiSeq2000

Number of raw reads reads1

56,770

7,164,496 (paired reads)

Total nucelotides (raw data)1

223,483,907

1,421,726,349

Mean read length (raw data)1

3,937

99

Total nucleotides (error-corrected data)

54,492,250

n.a.

Mean read length (error-corrected data)

1,902

n.a.

Pre-assembly error-rate2

1.3%

0.117%

Ambiguous bases post-assembly3

0%

0.12%

Assembled genome coverage

100%

90.59%

Average depth of coverage

320×

9,111×

Number of contigs

1

7

Total genome coverage (bp)

154,959

148,776

  1. Summary statistics for the assembly of the P. Micrantha chloroplast genome using PacBio RS and Illumina HiSeq2000 sequencing data.
  2. 1Trimmed Illumina reads.
  3. 2Error-corrected PacBio reads and raw Illumina reads.
  4. 3In comparison to the chloroplast consensus sequence.