From: An evaluation of the PacBio RS platform for sequencing and de novo assembly of a chloroplast genome
 | PacBio RS | Illumina HiSeq2000 |
---|---|---|
Number of raw reads reads1 | 56,770 | 7,164,496 (paired reads) |
Total nucelotides (raw data)1 | 223,483,907 | 1,421,726,349 |
Mean read length (raw data)1 | 3,937 | 99 |
Total nucleotides (error-corrected data) | 54,492,250 | n.a. |
Mean read length (error-corrected data) | 1,902 | n.a. |
Pre-assembly error-rate2 | 1.3% | 0.117% |
Ambiguous bases post-assembly3 | 0% | 0.12% |
Assembled genome coverage | 100% | 90.59% |
Average depth of coverage | 320× | 9,111× |
Number of contigs | 1 | 7 |
Total genome coverage (bp) | 154,959 | 148,776 |