Figure 5From: Influence of developmental stage and genotype on liver mRNA levels among wild, domesticated, and hybrid rainbow trout (Oncorhynchus mykiss)Hierarchical clustering of mRNA expression profiles in liver tissue of all individual fish, including 3D-PCA plot. a) hierarchical clustering using Euclidian distance measurement on all mRNAs with significantly different expression (P ≤ 0.05 and fold change ≥ 2, 165 mRNAs total, includes replicates) common between D relative to W groups. The X-axis is clustered by group (condition) and the Y-axis by normalised mRNA expression values. Clustering was done using all individual samples for age-matched wilds (Wa), size-matched wilds (Ws), domesticated (D) and wild-domesticated hybrid (W/D). The expression bar indicates the normalised expression level for each mRNA (intensity), which are represented by various colour intensities. b) 3D-PCA plot showing variation based on biological variables. Three-dimensional principal component plot with each axis explaining a certain percentage of variation among all samples (n = 24). X-axis: 12.91% Y-axis: 9.47% and Z-axis: 6.47%. Samples are coloured according to genotype assignment with age-matched wild (grey), size-matched wild (red), domesticated (blue) and wild-domesticated hybrid (reddish brown).Back to article page