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Table 2 Putative TF sites from southwestern blot based on estimated kDa of bands

From: PuF, an antimetastatic and developmental signaling protein, interacts with the Alzheimer’s amyloid-β precursor protein via a tissue-specific proximal regulatory element (PRE)

Banda

kDa

Nuclear extractsb

Corresponding TFc

I

92

NB, HeLa, HeLa+TPA

SP1

II

64

NB, HeLa, HeLa+TPA

R

III

60

PC12, HeLa, HeLa+TPA

USF1, TCERG1

IV

51

NB, HeLa

USF1, AP2, GATA1, GATA2, TCERG1

V

39

NB

GATA1, USF1, AP2, Pax4a, USF2

VI

33

NB, PC12, HeLa, HeLa + TPA

USF1, AP2, Msx1

VII

32

NB, PC12, HeLa, HeLa + TPA

USF1, AP2, Msx1

VIII

23

NB

BTEB2

  1. aBands named in bold face are described as “major” in Figure 2.
  2. bNuclear extracts for which the band appears in the southwestern.
  3. cTF correspondence is based on calculated band kDa ±10%.