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Table 3 Adjusted/normalized ELISA and EMSA signals in NB cells and extracts

From: PuF, an antimetastatic and developmental signaling protein, interacts with the Alzheimer’s amyloid-β precursor protein via a tissue-specific proximal regulatory element (PRE)

Variant

EMSAa

 
 

Band I

Band II

ELISAa

 

Rel. PRE = 0

g

Rel. PRE = 0

g

Rel. PRE = 1

g

PRE

0.00 ± 0.18

0.00 ± 0.82

0.00 ± 0.11

0.00 ± 0.82

1.00 ± 0.17

0.00 ± 0.82

M1

1.81c ± 0.24

3.24 ± 0.99/0.95

−1.76c ± 0.11

−4.86 ± 1.29/1.31

0.37 ± 0.05

−3.12 ± 0.92/0.96

M2

−1.33b ± 0.26

−2.39 ± 0.79/0.84

−0.43 ± 0.13

−1.18 ± 0.62/0.66

0.81 ± 0.12

−0.91 ± 0.60/0.63

M3

0.60 ± 0.27

1.07 ± 0.65/0.61

0.79b ± 0.23

2.19 ± 0.80/0.76

1.69b ± 0.13

3.40 ± 1.02/0.98

M4

0.40 ± 0.38

0.72 ± 0.62/0.59

−1.62b ± 0.09

−4.48 ± 1.21/1.23

0.89 ± 0.02

−0.52 ± 0.58/0.60

M5

−0.28 ± 0.21

−0.51 ± 0.58/0.60

0.07 ± 0.22

0.19 ± 0.58/0.57

0.34b ± 0.08

−3.24 ± 0.95/0.99

M7

0.27 ± 0.31

0.48 ± 0.60/0.58

−0.61 ± 0.12

−1.68 ± 0.68/0.73

0.16b ± 0.12

−4.14 ± 1.13/1.16

M7

1.00 ± 0.42

1.78 ± 0.74/0.70

0.87b ± 0.35

2.41 ± 0.84/0.79

1.04 ± 0.09

0.18 ± 0.58/0.57

 

F (p)

Ψ d

F (p)

Ψ

F (p)

Ψ

ANOVA

9.79 (< 0.01)

1.65 ± 0.82/0.62

26.54 (< 0.01)

2.72 ± 1.09/0.97

21.20 (< 0.01)

2.43 ± 1.01/0.88

  1. aData is mean ± standard error of the mean (SEM). EMSA is normalized to wildtype = 0. ELISA is relative to wildtype = 1. SEM reported as (x/y as upper/lower) is asymmetrical due to noncentrality of standardized effect size measures [43].
  2. bSignal significantly different from wildtype at p < 0.05.
  3. cELISA and EMSA signal both significantly different from wildtype at Bonferroni-adjusted p < 0.05.
  4. dΨ as estimate ± 95% confidence interval.
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BMC Genomics

ISSN: 1471-2164

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