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Table 4 Adjusted/normalized ELISA and EMSA signals in PC12 cells and extracts

From: PuF, an antimetastatic and developmental signaling protein, interacts with the Alzheimer’s amyloid-β precursor protein via a tissue-specific proximal regulatory element (PRE)

Variant

EMSAa

 
 

Band I

Band II

ELISAa

 

Rel. PRE = 0

g

Rel. PRE = 0

g

Rel. PRE = 1

g

PRE

0.00 ± 0.23

0.00 ± 0.82

0.00 ± 0.1

0.00 ± 0.82

1.00 ± 0.11/0.09

0.00 ± 0.82

M1

1.25 ± 0.92/0.78

1.77 ± 0.74/0.69

−1.38c ± 0.07

−5.56 ± 1.44/1.45

0.39c ± 0.02

−6.84 ± 1.73

M2

−0.91 ± 0.11

−1.43 ± 0.65/0.69

−0.14 ± 0.18

−0.56 ± 0.58/0.60

0.67 ± 0.05/0.04

−2.05 ± 0.74/0.78

M3

0.56 ± 0.07

0.84 ± 0.63/0.60

1.20b ± 0.14

4.82 ± 1.30/1.28

0.83 ± 0.18/0.12

−0.8 ± 0.59/0.62

M4

0.61 ± 0.48/0.44

0.91 ± 0.63/0.60

−1.04c ± 0.11

−4.20 ± 1.15/1.17

0.45c ± 0.01

−5.33 ± 1.39/1.4

M5

0.16 ± 0.12

0.25 ± 0.58/0.57

0.30 ± 0.07

1.23 ± 0.67/0.63

0.43b ± 0.04/0.03

−5.69 ± 1.47/1.48

M6

0.27 ± 0.06

0.41 ± 0.59/0.58

−0.25 ± 0.13

−1.01 ± 0.61/0.64

0.43b ± 0.01

−5.68 ± 1.47/1.48

M7

1.46 ± 0.58/0.52

2.04 ± 0.78/0.73

1.58b ± 0.19

6.36 ± 1.62

1.03 ± 0.03

0.12 ± 0.58/0.57

 

F (p)

Ψ d

F (p)

Ψ

F (p)

Ψ

ANOVA

4.23 (< 0.01)

1.09 ± 0.78/0.45

58.96 (< 0.01)

4.05 ± 1.49/1.42

29.96 (< 0.01)

2.89 ± 1.14/1.03

  1. aData is mean ± standard error of the mean (SEM). EMSA is normalized to wildtype = 0. ELISA is relative to wildtype = 1. SEM reported as (x/y as upper/lower) is asymmetrical due to noncentrality of standardized effect size measures [43].
  2. bSignal significantly different from wildtype at p < 0.05.
  3. cELISA and EMSA signal both significantly different from wildtype at Bonferroni-adjusted p < 0.05.
  4. dΨ as estimate ± 95% confidence interval.
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BMC Genomics

ISSN: 1471-2164

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