PuF, an antimetastatic and developmental signaling protein, interacts with the Alzheimer’s amyloid-β precursor protein via a tissue-specific proximal regulatory element (PRE)

Table 7 Natural SNP in the PRE

SNP	Sequence	Sites deleted	Sites created
rs201592736	5’-TCGGGTGCCGAGCGA GGTGGGCCGGATCAG-3’	AP2, GC Box (I)^a, GATA1, PuF	RARγ, ZEB1
rs200621906	5’-TCGGGTGCCGAGCGGGGTGGGCCA GATCAG-3’	AP2, GC Box (II), SP1 (II)^b	ENKTF1, GR/PR,
both	5’-TCGGGTGCCGAGCGA GGTGGGCCA GATCAG-3’	AP2, GC Box (both), PuF, SP1 (II)	ENKTF1, GR/PR, RARγ, ZEB1

^aTwo distinct GC boxes were identified in the wildtype PRE sequence and are designated as “I” and “II”.
^bTwo distinct SP1 sites in addition to the GC boxes were identified in the wildtype PRE sequence and are designated as “I” and “II”.

ISSN: 1471-2164